
( Brand: Ge Healthcare ), ( Manufacturer Part Number: 17-0854-02 ), ( Model: CAT 17-0852-01 ), ( Modified Item: No ), ( Intended Use/discipline: Biological Laboratory )
The Ge Healthcare Illustra Nap-25 Column Sephadex G-25 DNA Grade (17-0852-01) is a high-quality, ready-to-use, and disposable desalting and buffer exchange column designed for the purification and desalting of nucleic acids, including DNA. This column is part of the widely used and trusted Ge Healthcare Illustra Nap series, which is known for its excellent performance and reliability.
The 17-0852-01 column features a Sephadex G-25 resin, which is a cross-linked dextran gel that effectively removes salts, buffers, and small molecules from nucleic acids while retaining the nucleic acid itself. The gel has a pore size of 25 , which allows for the separation of DNA molecules based on their size and molecular weight, ensuring that the desalted DNA is of high purity and integrity.
The column has a total volume of 25 ml, with a bed volume of 10 ml, and a nominal diameter of 1 cm. This makes it an ideal size for a variety of applications, from small-scale research experiments to larger-scale purifications. The column is designed to be used with a vacuum manifold, which allows for easy and efficient loading, washing, and elution of the sample.
The Ge Healthcare Illustra Nap-25 Column Sephadex G-25 DNA Grade (17-0852-01) is compatible with a wide range of buffers and salts, including Tris, EDTA, and NaCl. It is also compatible with a variety of nucleic acid binding dyes, such as Ethidium Bromide and SYBR Green, making it easy to monitor the progress of the purification process.
The column comes in a sterile, ready-to-use format, and is stable for up to 12 months when stored at 2-8 C. It is also compatible with a variety of automated liquid handling systems, making it easy to integrate into laboratory workflows.
In summary, the Ge Healthcare Illustra Nap-25 Column Sephadex G-25 DNA Grade (17-0852-01) is a versatile and high-performance desalting and buffer exchange column that is ideal for the purification and desalting of nucleic acids, including DNA. Its easy-to-use format, wide compatibility with buffers and salts, and ability to be used with automated systems make it a valuable tool in any laboratory.
Additionally, Ge Healthcare Illustra Nap-25 Column Sephadex G-25 DNA Grade (17-0854-02) is also available, which is the same as 17-0852-01 but with a 50ml total volume, 20ml bed volume and 1.5cm nominal diameter. This column is ideal for larger scale purifications, while the 17-0852-01 is for smaller scale experiments.
Product: Ge Healthcare Illustra Nap-25 Columns Sephadex G25 DNA Cat 17-0852-01 and 17-0854-02
Pros:1. High-quality product: Ge Healthcare is a reputable company known for producing high-quality lab equipment. The Sephadex G25 DNA Columns are no exception.
2. Effective for DNA separation: The Sephadex G25 resin is specifically designed for the separation of DNA molecules based on size, making it an ideal tool for molecular biology research.
3. Reusable: The columns can be reused multiple times, making them a cost-effective option in the long run.
4. Easy to use: The columns are designed to be user-friendly, making them suitable for both experienced scientists and beginners.
Cons:1. Expensive: Compared to other DNA separation methods, such as agarose gel electrophoresis, the columns can be quite pricey.
2. Limited capacity: The columns have a limited capacity, which means they may not be suitable for large-scale DNA separations.
3. Requires specialized equipment: The columns require a vacuum pump and a fraction collector, which can add to the overall cost.
Conclusion:The Ge Healthcare Illustra Nap-25 Columns Sephadex G25 DNA Cat 17-0852-01 and 17-0854-02 are a high-quality and effective tool for DNA separation. However, their high cost and limited capacity may make them unsuitable for some researchers. If the benefits of using the columns outweigh the costs and limitations, then they would be a good investment. If not, alternative methods such as agarose gel electrophoresis may be more suitable. Ultimately, the decision should be based on the specific needs and resources of the research.
Specifications for use with application rapid and efficient purification of dna step-by-step protocol identifying the fixed volumes needed for calibration, washing sample elation-25 retail price: 275.